This comprehensive, fully updated text introduces the essential concepts of biochemical and bioprocess engineering to students in chemical engineering and those pursuing courses in related disciplines. The authors first review the relevant fundamentals of biochemistry, microbiology, and molecular biology, introducing key principles that enable bioprocess engineers to achieve consistent control over biological activity. The text then reflects the advances that are transforming the field, ranging from genetic sequencing to new techniques for producing proteins from recombinant DNA. It introduces techniques with broad applications to the production of pharma-ceuticals, biologics, and commodities. It also covers medical applications such as tissue engineering and gene therapy and those used for solving critical environmental problems. Special Features Covers essential biological basics: microorganism structure and functions, major metabolic pathways, enzymes, microbial genetics, kinetics, and stoichiometry of growth. New coverage of posttranslational processing of proteins-an essential technique for manu-facturing therapeutic proteins. Explains concepts, techniques and applications of functional genomics and cellular engineering. Applies bioprocess engineering approaches to biomedical applications. In-depth coverage of animal cell culture processes. New coverage of noncarbohydrate meta-bolism. Discusses regulatory constraints on bio-process design and development.

Table of Contents

Part 1: INTRODUCTION- What is a Bioprocess Engineer?

Part 2: THE BASICS OF BIOLOGY: AN ENGINEER'S PERSPECTIVE
An Overview of Biological Basics. Enzymes.
How Cells Work. Major Metabolic Pathways.
How Cells Grow.
Stoichiometry of Microbial Growth and Product Formation.
How Cellular Information is Altered.

Part 3: ENGINEERING PRINCIPLES FOR BIOPROCESSES-
Operating Considerations for Bioreactors for Suspension and Immobilized Cultures.
Selection, Scale-Up, Operation, and Control of Bioreactors.
Recovery and Purification of Products.

Part 4: APPLICATIONS TO NONCONVENTIONAL BIOLOGICAL SYSTEMS-
Bioprocess Considerations in using Animal Cell Cultures.
Bioprocess Considerations in using Plant Cell Cultures.
Utilizing Genetically Engineered Organisms.
Medical Applications of Bioprocess Engineering.
Mixed Cultures. Epilogue.

Appendix:
Traditional Industrial Bioprocesses.