Protein Engineering: Principles and Practice

This up-to-the-minute text presents a systematic approach to protein engineering from both fundamental and operational perspectives. Contains a thorough introduction to protein biophysics via chapters dealing with experimental and theoretical determination of protein structure. Discusses each of the various expression systems currently available along with their advantages and limitations. Also includes the difficulty in achieving correctly folded and functional proteins, specific examples of protein engineering use in construction of synthetic metal binding sites, the manufacture of tissue plasminogen and the generation of therapeutic antibodies.