RNA Synthetic Biology: Fundamentals and Applications

This book discusses the fundamental principles and applications of RNA synthetic biology. The initial section of the book provides fundamental concepts, including RNA synthetic biology, the use of computational biology tools for RNA design, the SELEX (Systematic Evolution of Ligands by EXponential enrichment) method, artificial intelligence for the design and prediction of RNA circuits, RNA structure prediction and folding algorithms, non-coding RNA, and in vitro selection of functional RNA. This book also highlights the practical applications of RNA synthetic biology, offering design, construction, and testing of riboswitches, riboregulators, RNA-based biosensors, CRISPR for RNA detection, RNA editing, application in nanotechnology, RNA economics, and ethics and regulatory issues. This book is intended for researchers and students of molecular biology, genetics, synthetic biology, and RNA biology.

Key Features:

• Presents the fundamental principles and applications of RNA synthetic biology
• Reviews the applications of computational tools and artificial intelligence in the design, prediction, and analysis of RNAs and RNA circuits
• Explores the role of RNA modifications on function and the regulatory roles of non-coding RNAs
• Examines CRISPR-based RNA detection and RNA-based therapeutics for genetic diseases, cancer, and infectious diseases
• Covers the design and utilization of riboswitches, riboregulators, RNA sensors, and RNA nanotechnology
• Introduces design and applications of RNA aptamers and their use as diagnostic tools, therapeutics, and molecular probes