Drosophila Oogenesis: Methods and Protocols (2023)

1. The Vast Utility of Drosophila Oogenesis

Michelle S. Giedt and Tina L. Tootle

2. Visualization and Quantification of Drosophila Larval Ovaries

Alicia E. Rosales-Nieves, Miriam Marín-Menguiano, Alejandro Campoy-Lopez, and Acaimo González-Reyes

3. Dissection, Fixation, and Standard Staining of Adult Drosophila Ovaries

Julie A. Merkle

4. Utilizing the FLP-Out System for Clonal RNAi Analysis in the Adult Drosophila Ovary

Daniel N. Phipps, Amanda M. Powell, and Elizabeth T. Ables

5. Analysis of Physiological Control of Adult Drosophila Oogenesis by Inter-Organ Communication

Lesley N. Weaver

6. Immunohistochemical Analysis of Nuclear Lamina Structures in the Drosophila Ovary Using CRISPR-Tagged Genes

Tingting Duan, Felipe Rodriguez-Tirado, and Pamela K. Geyer

7. Visualizing Fusome Morphology via Tubulin Immunofluorescence in Drosophila Ovarian Germ Cells

Anna E. Williams and Elizabeth T. Ables

8. Tracking Follicle Cell Development

Adrianna Soriano, Christopher Petit, Savannah Ryan, and Jennifer C. Jemc

9. Optimized Fixation and Phalloidin Staining of Basally Localized F-Actin Networks in Collectively Migrating Follicle Cells

Mitchell T. Anderson, Kristin Sherrard, and Sally Horne-Badovinac

10. Quantitative Image Analysis of Dynamic Cell Behaviors during Border Cell Migration

Yujun Chen, Nirupama Kotian, and Jocelyn A. McDonald

11. Live Imaging of Nurse Cell Behavior in Late Stages of Drosophila Oogenesis

Jonathan A. Jackson, Jasmin Imran Alsous, and Adam C. Martin

12. Visualizing Lipid Droplets in Drosophila Oogenesis

Roger P. White and Michael A. Welte

13. Assessing Ovulation in Drosophila melanogaster

Andrew Beard, Rebecca Oramas, and Jianjun Sun

14. A Low-Tech Flow Chamber for Live Imaging of Drosophila Egg Chambers during Drug Treatments

Allison L. Zajac, Audrey M. Williams, and Sally Horne-Badovinac

15. Detection and Assessment of Wolbachia pipientis Infection

Lindsay B.M. Nevalainen and Irene L.G. Newton

16. Measuring Transposable Element Activity in Adult Drosophila Ovaries

Anikó Szabó, Péter Borkúti, Zoltán Kovács, Ildikó Kristó, Csilla Abonyi, and Péter Vilmos

17. Preparation of Drosophila Ovarioles for Single-Cell RNA Sequencing

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